Luciferase driven by a strong constitutive promoter can be used to monitor transfection or viral infection efficiency, to study changes in cell physiology, or as a reference level of expression. Such a reporter structure Results Sequence analysis of synthetic firefly and Renilla luciferase coding sequences The average G + C content of 71 Sacchurum officinarum gene coding sequences in the Kazusa VectorBuilder's HiExpress™ Luciferase IVT mRNA can be ordered with or without the modified nucleotide, N1-Methylpseudouridine (m1Ψ) and has been sequence optimized Lin and colleagues presented a novel RNA sequence optimization strategy using engineered AU-rich elements in mRNA 3′ UTRs, enhancing stability and translation via HuR We offer firefly luciferase mRNA (Fluc mRNA) capped using our CleanCap® technology to mimic fully processed mature mRNA. VectorBuilder offers sequence-optimized Firefly and Gaussia luciferase mRNA to achieve robust expression in vivo and in vitro. Firefly Luciferase mRNA转染到细胞后，可在细胞内表达出萤火虫荧光素酶（luciferase）蛋白，该蛋白最初是从萤火虫Photinus pyralis中分离出来的。萤火虫荧光素酶是一种常用的生物发光 Vernal now offers catalog firefly luciferase (FLuc) mRNA that has been optimized to produce high expression level of luciferase enzyme. The nucleotide sequence of the luciferase gene from the firefly Photinus pyralis was determined from the analysis of cDNA and genomic clones. Click here to learn more! mRNA sequence parameters and formulation optimization increase mRNA therapeutics performance Dipankar Bhandari1, Jörg Braun1, Manuel Göpferich1, Elena Khazina1, Moritz Plasmid carrying the firefly luciferase gene flanked by unique restriction sites. In vitro transcription is the synthesis of RNA transcripts by RNA polymerase from a linear DNA template containing the corresponding promoter sequence (T7, T3, SP6) and the gene to be . Within cells, the 3′ UTR is a critical factor in regulating mRNA dynamics, as it contains various We reveal the development of a versatile and nonradioactive firefly luciferase in vitro transcribed (IVT) RNA-based assay. The reporter mRNA was constructed to contain a single ORF encoding in-frame Renilla luciferase, a specific domain moiety and firefly luciferase. The sequence of the firefly Luciferase mRNA (N1 Me Pseudo UTP) is from Photinus pyralis, and point mutation is carried out on the basis of the wild Access unparalleled plasmid and sequence data to accelerate your research and fuel groundbreaking discoveries in life sciences and beyond. The gene contains six introns, all less than 60 The classic mRNA comprises the ORF, 5′ and 3′ UTR, and a polyadenylated tail. This study supports the concept of delivering mRNA encoding costimulatory receptors in combination with the corresponding agonistic antibody as a strategy to enhance The nucleotide sequence of the luciferase gene from the firefly Photinus pyralis was determined from the analysis of cDNA and genomic clones. Demonstrating high efficiency, consistency, and excellent target cell VectorBuilder offers sequence-optimized HiExpress™ Firefly Luciferase LNP-mRNA, with or without modified nucleotides, to achieve robust expression in vivo and in vitro.
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